CHARACTERIZATION OF LNP LIBRARY. In this study, ionizable lipid nanoparticles (LNPs) were investigated for mRNA delivery to T cells. LNPs were selected because they have been shown to deliver mRNA intracellularly with high potency and low cytotoxicity to a range of cell and tissue targets in vivo and ex vivo.Most recently, LNPs …
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Transposon-mediated iPS cells derived from patients suffering from either sickle cell disease caused by a β-globin gene mutation or Huntington's disease caused by trinucleotide repeat expansions in the Huntingtin gene were successfully used for gene editing ... World J Stem Cells. 2015; 7:315–328. [PMC free article] [Google Scholar] 107.
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Delivery of genetic material to cells is an integral tool to analyze and reveal the genetic interventions in normal cellular processes and differentiation, disease development and for gene therapy. ... Cold atmospheric plasma mediated cell membrane permeation and gene delivery-empirical interventions and pertinence Adv Colloid Interface Sci ...
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This model is consistent with data showing that (1) Wnts can signal in an autocrine manner, (2) Wnt and WLS can transfer together across synapses on lipid vesicles such as exosomes, and (3) WLS and Wnt are …
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Methods: Overexpression and knockdown of Nr1d1 in Sca-1 + CD31 - cells and mouse cardiac myocyte (MCM) cell lines were performed by lentiviral transduction. The effects of Nr1d1 abundance on cell differentiation, proliferation, apoptosis, cell cycle, and transcriptomics were evaluated. Moreover, binding of Nr1d1 to the promoter region of …
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Mycoplasma contamination in cell culture affects the properties of cell lines. Gold standard detection by microbiological culture takes days and requires specialists. The polymerase chain reaction and loop-mediated isothermal amplification (LAMP) are fast molecular options, but LAMP only requires one heating block for DNA amplification. This …
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The treatment of human pulmonary artery smooth muscle cells or human pulmonary artery endothelial cells with recombinant SARS-CoV-2 spike protein S1 subunit (Val16 – Gln690) at 10 ng/ml (0.13 nM) caused an activation of MEK phosphorylation. The activation kinetics was transient with a peak at 10 min. The recombinant protein that …
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Although for a long time lysosomes were considered unable to contribute to the selective degradation of individual proteins, the discovery of chaperone-mediated autophagy (CMA) changed this notion. Here, we review the characteristics that set CMA apart from other types of lysosomal degradation and the subset of molecules that confer cells the ...
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ZVAD-FMK-mediated global pan-caspase inhibition and the apparent absence of cleaved caspase-3 in treated cells indicated that the death did not involve …
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This vesicle-mediated cell detoxification process provides a strategy to increase plant resistance against different toxin-associated diseases and to reduce the mycotoxin contamination in food and ...
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Ex vivo assays exist for assessing cytotoxicity of CAR-T cells, but each assay has limitations. For example, the chromium release assay, where cells are …
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In vivo Eed isoform 2, is expressed in cancer cells and undifferentiated embryonic stem cells, and associates with the histone deacetylase Sirt1 in the so-called PRC4 required for the methylation of H1K26. 42, 127, 128 In the PRC1 world, the CBX2 subunit undergoes alternative splicing due to different polyadenylation sites, arising two ...
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Chaperone-mediated autophagy: a unique way to enter the lysosome world. Trends Cell Biol 2012; 22 :407–417. Article CAS PubMed PubMed Central Google Scholar
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In our first article, Immunology 101: Innate and Adaptive Immunity Explained, we briefly introduced the concepts of the humoral immune response and the cell-mediated immune response as components of adaptive immunity. As a quick recall, adaptive immunity (also known as "acquired immunity", since your body needs to …
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English . Term. Definition. cell-mediated immune response. type of immune response in which T cells destroy cells that are infected with viruses. cytotoxic T cell. cell that destroys virus-infected cells and some cancer cells. helper T cell. manager of the immune response; secretes cytokines; may remain as memory cell.
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Immunity, cell mediated The immune system is a network of cells and organs that work together to protect the body from infectious organisms. Many different types of organisms such as bacteria, viruses, fungi, and parasites are capable of entering the human body and causing disease. It is the immune system's job to recognize these agents as foreign …
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The immune system has tailored its effector functions to optimally respond to distinct species of microbes. Based on emerging knowledge on the different effector T-cell and innate lymphoid cell (ILC) lineages, it is clear that the innate and adaptive immune systems converge into 3 major kinds of cell-mediated effector immunity, which we propose to …
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The role of effector T cells in cell-mediated and humoral immune responses to representative pathogens. Cell-mediated immune responses involve the destruction of infected cells by cytotoxic T cells, or the …
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Cell-mediated immunity is directed primarily microbes that survive in phagocytes and microbes that infect non-phagocytic cells. It is most effective in destroying virus-infected cells, intracellular bacteria, …
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Reperfusion may induce additional cell death in patients with acute myocardial infarction receiving primary angioplasty or thrombolysis. Altered intracellular Ca (2+) handling was initially considered an essential mechanism of reperfusion-induced cardiomyocyte death. However, more recent studies have demonstrated the importance …
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For the cell-to-cell transmission assay, target cells were labelled with CMRA dye (1:1,000, C34551, Thermo Fisher Scientific, emission maximum at 575 nm) for 30 min, washed with PBS three times ...
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Most paramyxoviruses enter epithelial cells of the airway using sialic acid as a receptor and cause only mild disease. However, others cross the epithelial ba … Receptor-mediated cell entry of paramyxoviruses: Mechanisms, and consequences for tropism and pathogenesis J Biol Chem. 2020 Feb 28;295(9):2771-2786. doi: …
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Aim: To investigate whether Yiguanjian decoction (YGJ) has an anti-liver cirrhotic effect and whether it regulates hepatic stem cell differentiation. Methods: A rat model of liver cirrhosis was established via subcutaneous injection of carbon tetrachloride (CCl 4) for 8 wk.From the beginning of the ninth week, the rats received 2 …
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Activating immunogenic cell death (ICD) holds promise in enhancing tumor cell immunogenicity, stimulating immune amplification response, improving drug sensitivity, and eliminating tumors. Nanotechnology-enabled ICD has emerged as a compelling therapeutic strategy for augmenting cancer immunotherapy. Nanoparticles possess …
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Here, we performed a large-scale immune cell gene-expression analysis, together with whole-genome sequence analysis. Our dataset consists of 28 distinct immune cell …
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Biologists have studied the process of directed migration for more than a century, but many mysteries remain about how this process works at a mechanistic …
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The immune system is divided into nonspecific (innate) and specific (adaptive) responses. Nonspecific defenses, like skin and stomach acid, block pathogens indiscriminately. Specific …
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This timeline article pays tribute to the late James Fred 'Paulo' Dice, whose vision of selective protein degradation in lysosomes led to the discovery of chaperone-mediated autophagy. A little ...
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While SARS1-S-mediated cell-cell fusion was also weakly activated when ACE2 was expressed alone, activation was much higher in the presence of TMPRSS2, indicating stronger dependence of SARS1-S on TMPRSS2, compatible with the monobasic S1/S2 cleavage site in the SARS-CoV spike protein. Surprisingly, we even observed …
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The Immunological Basis of T-Cell Mediated Rejection. T-cell mediated rejection (TCMR, also previously known as "acute cellular rejection") occurs most commonly in the early post-transplant period and is generally amenable to treatment with immunosuppression ().It typically presents with non-specific clinical symptoms and …
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